Transferring:Article Title: Gradient-mixing LEGO robots for purifying DNA origami nanostructures of multiple components by rate-zonal centrifugation
Article Snippet: Concentration gradient 50 × TAE buffer, pH 8.3 (VWR Life Science, cat. no. 75800-940) Magnesium chloride hexahydrate (Sigma-Aldrich, cat. no. M9272) Glycerol (VWR Life Science, cat. no. BDH1172) Sucrose (Sigma-Aldrich, product no. S 0389) DNA origami folding 50× TAE buffer, pH 8.3 (VWR Life Science, cat. no. 75800-940) Magnesium chloride hexahydrate (Sigma-Aldrich, cat. no. M9272) Staple strands (Integrated DNA Technique Inc.) ssDNA scaffold p8064 (Tilibit Nanosystem, product no. M1-51) Agarose Gel Electrophoresis SYBR gold DNA gel stain (Thermo Fisher Scientific, cat no. S11494) DNA gel loading dye (Thermo Fisher Scientific, cat no. R0631) 1 kb DNA ladder (Gold Biotechnology Inc., cat. no. D010-500) Agarose tablets (EURx, cat. no. E0305-01) .. General P2L Eppendrof pipette (Gilson, SKU FA10001M) P10L Eppendrof pipette (Gilson, SKU FA10002M) P200L Eppendrof pipette (Gilson, SKU FA10005M) P1000L Eppendrof pipette (Gilson, SKU FA10006M) EXPERT tips E200 Tipack (Gilson, SKU F1733002) EXPERT Tips E1000 XL Tipack (Gilson, SKU F1735002) Milli-Q® Direct Water Purification System (Milipore Sigma, cat. no. ZR0Q016WW) Density gradient preparation EV3 LEGO Mindstorm (LEGO, item no. 31313) Ultimaker 3 3D printer (part no. 9671) PLA 3D printing materials 1 mL syringe (Becton Dickinson, cat. no. 309628) DNA origami preparation Thermocycler, MiniAmpTM Thermal Cycler REX (Thermo Fisher Scientific, cat No. A38080) PCR tubes (USA Scientific, cat. no. 1402-8120) 100 kD amicon filter (Milipore, cat. no. UFC510024) Benchmark MC-12 High Speed Microcentrifuge (Marshall Scientific, item no. C1612) Rate-zonal centrifugation Beckman TLS 55 swinging bucket rotor (Beckman Coulter, part no. 346936) Polycarbonate centrifuge tube (Beckman Coulter, part no. 343778) Optima TLX ultracentrifuge (Beckman Coulter, part no. 361545) Longneck gel-loading tips (Fisher Scientific, cat. no. 02-707-81) Safe Imager 2.0 Blue Light transilluminators (Invitrogen, cat. no. G6600) Gel electrophoresis 250 mL Erlenmeyer flask (Karter Scientific, part no. 214U2) External gel cast (Thermo Fisher Scientific, cat. no. B2-CST) OwlTM easyCastTM B2 Mini Gel Electrophoresis Systems (Thermo Fisher Scientific, cat. no. B2-BP) Bio-Rad Molecular Imager Gel Doc XR+ (Bio-Rad, part. no. 1708195EDU) Atomic force microscopy MultiMode 8-HR AFM (Bruker) ScanAsyst-Fluid+probes cantilever (Bruker) Glass probe holder MTFML-V2 (Bruker) V1 AFM mica discs, 10mm (Ted Pella Inc., product no. 50-10) AFM/STM Metal Specimen Discs, 12mm (Ted Pella Inc., product no. 16208) ..
Purification:Article Title: Gradient-mixing LEGO robots for purifying DNA origami nanostructures of multiple components by rate-zonal centrifugation
Article Snippet: Concentration gradient 50 × TAE buffer, pH 8.3 (VWR Life Science, cat. no. 75800-940) Magnesium chloride hexahydrate (Sigma-Aldrich, cat. no. M9272) Glycerol (VWR Life Science, cat. no. BDH1172) Sucrose (Sigma-Aldrich, product no. S 0389) DNA origami folding 50× TAE buffer, pH 8.3 (VWR Life Science, cat. no. 75800-940) Magnesium chloride hexahydrate (Sigma-Aldrich, cat. no. M9272) Staple strands (Integrated DNA Technique Inc.) ssDNA scaffold p8064 (Tilibit Nanosystem, product no. M1-51) Agarose Gel Electrophoresis SYBR gold DNA gel stain (Thermo Fisher Scientific, cat no. S11494) DNA gel loading dye (Thermo Fisher Scientific, cat no. R0631) 1 kb DNA ladder (Gold Biotechnology Inc., cat. no. D010-500) Agarose tablets (EURx, cat. no. E0305-01) .. General P2L Eppendrof pipette (Gilson, SKU FA10001M) P10L Eppendrof pipette (Gilson, SKU FA10002M) P200L Eppendrof pipette (Gilson, SKU FA10005M) P1000L Eppendrof pipette (Gilson, SKU FA10006M) EXPERT tips E200 Tipack (Gilson, SKU F1733002) EXPERT Tips E1000 XL Tipack (Gilson, SKU F1735002) Milli-Q® Direct Water Purification System (Milipore Sigma, cat. no. ZR0Q016WW) Density gradient preparation EV3 LEGO Mindstorm (LEGO, item no. 31313) Ultimaker 3 3D printer (part no. 9671) PLA 3D printing materials 1 mL syringe (Becton Dickinson, cat. no. 309628) DNA origami preparation Thermocycler, MiniAmpTM Thermal Cycler REX (Thermo Fisher Scientific, cat No. A38080) PCR tubes (USA Scientific, cat. no. 1402-8120) 100 kD amicon filter (Milipore, cat. no. UFC510024) Benchmark MC-12 High Speed Microcentrifuge (Marshall Scientific, item no. C1612) Rate-zonal centrifugation Beckman TLS 55 swinging bucket rotor (Beckman Coulter, part no. 346936) Polycarbonate centrifuge tube (Beckman Coulter, part no. 343778) Optima TLX ultracentrifuge (Beckman Coulter, part no. 361545) Longneck gel-loading tips (Fisher Scientific, cat. no. 02-707-81) Safe Imager 2.0 Blue Light transilluminators (Invitrogen, cat. no. G6600) Gel electrophoresis 250 mL Erlenmeyer flask (Karter Scientific, part no. 214U2) External gel cast (Thermo Fisher Scientific, cat. no. B2-CST) OwlTM easyCastTM B2 Mini Gel Electrophoresis Systems (Thermo Fisher Scientific, cat. no. B2-BP) Bio-Rad Molecular Imager Gel Doc XR+ (Bio-Rad, part. no. 1708195EDU) Atomic force microscopy MultiMode 8-HR AFM (Bruker) ScanAsyst-Fluid+probes cantilever (Bruker) Glass probe holder MTFML-V2 (Bruker) V1 AFM mica discs, 10mm (Ted Pella Inc., product no. 50-10) AFM/STM Metal Specimen Discs, 12mm (Ted Pella Inc., product no. 16208) ..
Proximity Ligation Assay:Article Title: Gradient-mixing LEGO robots for purifying DNA origami nanostructures of multiple components by rate-zonal centrifugation
Article Snippet: Concentration gradient 50 × TAE buffer, pH 8.3 (VWR Life Science, cat. no. 75800-940) Magnesium chloride hexahydrate (Sigma-Aldrich, cat. no. M9272) Glycerol (VWR Life Science, cat. no. BDH1172) Sucrose (Sigma-Aldrich, product no. S 0389) DNA origami folding 50× TAE buffer, pH 8.3 (VWR Life Science, cat. no. 75800-940) Magnesium chloride hexahydrate (Sigma-Aldrich, cat. no. M9272) Staple strands (Integrated DNA Technique Inc.) ssDNA scaffold p8064 (Tilibit Nanosystem, product no. M1-51) Agarose Gel Electrophoresis SYBR gold DNA gel stain (Thermo Fisher Scientific, cat no. S11494) DNA gel loading dye (Thermo Fisher Scientific, cat no. R0631) 1 kb DNA ladder (Gold Biotechnology Inc., cat. no. D010-500) Agarose tablets (EURx, cat. no. E0305-01) .. General P2L Eppendrof pipette (Gilson, SKU FA10001M) P10L Eppendrof pipette (Gilson, SKU FA10002M) P200L Eppendrof pipette (Gilson, SKU FA10005M) P1000L Eppendrof pipette (Gilson, SKU FA10006M) EXPERT tips E200 Tipack (Gilson, SKU F1733002) EXPERT Tips E1000 XL Tipack (Gilson, SKU F1735002) Milli-Q® Direct Water Purification System (Milipore Sigma, cat. no. ZR0Q016WW) Density gradient preparation EV3 LEGO Mindstorm (LEGO, item no. 31313) Ultimaker 3 3D printer (part no. 9671) PLA 3D printing materials 1 mL syringe (Becton Dickinson, cat. no. 309628) DNA origami preparation Thermocycler, MiniAmpTM Thermal Cycler REX (Thermo Fisher Scientific, cat No. A38080) PCR tubes (USA Scientific, cat. no. 1402-8120) 100 kD amicon filter (Milipore, cat. no. UFC510024) Benchmark MC-12 High Speed Microcentrifuge (Marshall Scientific, item no. C1612) Rate-zonal centrifugation Beckman TLS 55 swinging bucket rotor (Beckman Coulter, part no. 346936) Polycarbonate centrifuge tube (Beckman Coulter, part no. 343778) Optima TLX ultracentrifuge (Beckman Coulter, part no. 361545) Longneck gel-loading tips (Fisher Scientific, cat. no. 02-707-81) Safe Imager 2.0 Blue Light transilluminators (Invitrogen, cat. no. G6600) Gel electrophoresis 250 mL Erlenmeyer flask (Karter Scientific, part no. 214U2) External gel cast (Thermo Fisher Scientific, cat. no. B2-CST) OwlTM easyCastTM B2 Mini Gel Electrophoresis Systems (Thermo Fisher Scientific, cat. no. B2-BP) Bio-Rad Molecular Imager Gel Doc XR+ (Bio-Rad, part. no. 1708195EDU) Atomic force microscopy MultiMode 8-HR AFM (Bruker) ScanAsyst-Fluid+probes cantilever (Bruker) Glass probe holder MTFML-V2 (Bruker) V1 AFM mica discs, 10mm (Ted Pella Inc., product no. 50-10) AFM/STM Metal Specimen Discs, 12mm (Ted Pella Inc., product no. 16208) ..
Polymerase Chain Reaction:Article Title: Gradient-mixing LEGO robots for purifying DNA origami nanostructures of multiple components by rate-zonal centrifugation
Article Snippet: Concentration gradient 50 × TAE buffer, pH 8.3 (VWR Life Science, cat. no. 75800-940) Magnesium chloride hexahydrate (Sigma-Aldrich, cat. no. M9272) Glycerol (VWR Life Science, cat. no. BDH1172) Sucrose (Sigma-Aldrich, product no. S 0389) DNA origami folding 50× TAE buffer, pH 8.3 (VWR Life Science, cat. no. 75800-940) Magnesium chloride hexahydrate (Sigma-Aldrich, cat. no. M9272) Staple strands (Integrated DNA Technique Inc.) ssDNA scaffold p8064 (Tilibit Nanosystem, product no. M1-51) Agarose Gel Electrophoresis SYBR gold DNA gel stain (Thermo Fisher Scientific, cat no. S11494) DNA gel loading dye (Thermo Fisher Scientific, cat no. R0631) 1 kb DNA ladder (Gold Biotechnology Inc., cat. no. D010-500) Agarose tablets (EURx, cat. no. E0305-01) .. General P2L Eppendrof pipette (Gilson, SKU FA10001M) P10L Eppendrof pipette (Gilson, SKU FA10002M) P200L Eppendrof pipette (Gilson, SKU FA10005M) P1000L Eppendrof pipette (Gilson, SKU FA10006M) EXPERT tips E200 Tipack (Gilson, SKU F1733002) EXPERT Tips E1000 XL Tipack (Gilson, SKU F1735002) Milli-Q® Direct Water Purification System (Milipore Sigma, cat. no. ZR0Q016WW) Density gradient preparation EV3 LEGO Mindstorm (LEGO, item no. 31313) Ultimaker 3 3D printer (part no. 9671) PLA 3D printing materials 1 mL syringe (Becton Dickinson, cat. no. 309628) DNA origami preparation Thermocycler, MiniAmpTM Thermal Cycler REX (Thermo Fisher Scientific, cat No. A38080) PCR tubes (USA Scientific, cat. no. 1402-8120) 100 kD amicon filter (Milipore, cat. no. UFC510024) Benchmark MC-12 High Speed Microcentrifuge (Marshall Scientific, item no. C1612) Rate-zonal centrifugation Beckman TLS 55 swinging bucket rotor (Beckman Coulter, part no. 346936) Polycarbonate centrifuge tube (Beckman Coulter, part no. 343778) Optima TLX ultracentrifuge (Beckman Coulter, part no. 361545) Longneck gel-loading tips (Fisher Scientific, cat. no. 02-707-81) Safe Imager 2.0 Blue Light transilluminators (Invitrogen, cat. no. G6600) Gel electrophoresis 250 mL Erlenmeyer flask (Karter Scientific, part no. 214U2) External gel cast (Thermo Fisher Scientific, cat. no. B2-CST) OwlTM easyCastTM B2 Mini Gel Electrophoresis Systems (Thermo Fisher Scientific, cat. no. B2-BP) Bio-Rad Molecular Imager Gel Doc XR+ (Bio-Rad, part. no. 1708195EDU) Atomic force microscopy MultiMode 8-HR AFM (Bruker) ScanAsyst-Fluid+probes cantilever (Bruker) Glass probe holder MTFML-V2 (Bruker) V1 AFM mica discs, 10mm (Ted Pella Inc., product no. 50-10) AFM/STM Metal Specimen Discs, 12mm (Ted Pella Inc., product no. 16208) ..
Centrifugation:Article Title: Gradient-mixing LEGO robots for purifying DNA origami nanostructures of multiple components by rate-zonal centrifugation
Article Snippet: Concentration gradient 50 × TAE buffer, pH 8.3 (VWR Life Science, cat. no. 75800-940) Magnesium chloride hexahydrate (Sigma-Aldrich, cat. no. M9272) Glycerol (VWR Life Science, cat. no. BDH1172) Sucrose (Sigma-Aldrich, product no. S 0389) DNA origami folding 50× TAE buffer, pH 8.3 (VWR Life Science, cat. no. 75800-940) Magnesium chloride hexahydrate (Sigma-Aldrich, cat. no. M9272) Staple strands (Integrated DNA Technique Inc.) ssDNA scaffold p8064 (Tilibit Nanosystem, product no. M1-51) Agarose Gel Electrophoresis SYBR gold DNA gel stain (Thermo Fisher Scientific, cat no. S11494) DNA gel loading dye (Thermo Fisher Scientific, cat no. R0631) 1 kb DNA ladder (Gold Biotechnology Inc., cat. no. D010-500) Agarose tablets (EURx, cat. no. E0305-01) .. General P2L Eppendrof pipette (Gilson, SKU FA10001M) P10L Eppendrof pipette (Gilson, SKU FA10002M) P200L Eppendrof pipette (Gilson, SKU FA10005M) P1000L Eppendrof pipette (Gilson, SKU FA10006M) EXPERT tips E200 Tipack (Gilson, SKU F1733002) EXPERT Tips E1000 XL Tipack (Gilson, SKU F1735002) Milli-Q® Direct Water Purification System (Milipore Sigma, cat. no. ZR0Q016WW) Density gradient preparation EV3 LEGO Mindstorm (LEGO, item no. 31313) Ultimaker 3 3D printer (part no. 9671) PLA 3D printing materials 1 mL syringe (Becton Dickinson, cat. no. 309628) DNA origami preparation Thermocycler, MiniAmpTM Thermal Cycler REX (Thermo Fisher Scientific, cat No. A38080) PCR tubes (USA Scientific, cat. no. 1402-8120) 100 kD amicon filter (Milipore, cat. no. UFC510024) Benchmark MC-12 High Speed Microcentrifuge (Marshall Scientific, item no. C1612) Rate-zonal centrifugation Beckman TLS 55 swinging bucket rotor (Beckman Coulter, part no. 346936) Polycarbonate centrifuge tube (Beckman Coulter, part no. 343778) Optima TLX ultracentrifuge (Beckman Coulter, part no. 361545) Longneck gel-loading tips (Fisher Scientific, cat. no. 02-707-81) Safe Imager 2.0 Blue Light transilluminators (Invitrogen, cat. no. G6600) Gel electrophoresis 250 mL Erlenmeyer flask (Karter Scientific, part no. 214U2) External gel cast (Thermo Fisher Scientific, cat. no. B2-CST) OwlTM easyCastTM B2 Mini Gel Electrophoresis Systems (Thermo Fisher Scientific, cat. no. B2-BP) Bio-Rad Molecular Imager Gel Doc XR+ (Bio-Rad, part. no. 1708195EDU) Atomic force microscopy MultiMode 8-HR AFM (Bruker) ScanAsyst-Fluid+probes cantilever (Bruker) Glass probe holder MTFML-V2 (Bruker) V1 AFM mica discs, 10mm (Ted Pella Inc., product no. 50-10) AFM/STM Metal Specimen Discs, 12mm (Ted Pella Inc., product no. 16208) ..
Nucleic Acid Electrophoresis:Article Title: Gradient-mixing LEGO robots for purifying DNA origami nanostructures of multiple components by rate-zonal centrifugation
Article Snippet: Concentration gradient 50 × TAE buffer, pH 8.3 (VWR Life Science, cat. no. 75800-940) Magnesium chloride hexahydrate (Sigma-Aldrich, cat. no. M9272) Glycerol (VWR Life Science, cat. no. BDH1172) Sucrose (Sigma-Aldrich, product no. S 0389) DNA origami folding 50× TAE buffer, pH 8.3 (VWR Life Science, cat. no. 75800-940) Magnesium chloride hexahydrate (Sigma-Aldrich, cat. no. M9272) Staple strands (Integrated DNA Technique Inc.) ssDNA scaffold p8064 (Tilibit Nanosystem, product no. M1-51) Agarose Gel Electrophoresis SYBR gold DNA gel stain (Thermo Fisher Scientific, cat no. S11494) DNA gel loading dye (Thermo Fisher Scientific, cat no. R0631) 1 kb DNA ladder (Gold Biotechnology Inc., cat. no. D010-500) Agarose tablets (EURx, cat. no. E0305-01) .. General P2L Eppendrof pipette (Gilson, SKU FA10001M) P10L Eppendrof pipette (Gilson, SKU FA10002M) P200L Eppendrof pipette (Gilson, SKU FA10005M) P1000L Eppendrof pipette (Gilson, SKU FA10006M) EXPERT tips E200 Tipack (Gilson, SKU F1733002) EXPERT Tips E1000 XL Tipack (Gilson, SKU F1735002) Milli-Q® Direct Water Purification System (Milipore Sigma, cat. no. ZR0Q016WW) Density gradient preparation EV3 LEGO Mindstorm (LEGO, item no. 31313) Ultimaker 3 3D printer (part no. 9671) PLA 3D printing materials 1 mL syringe (Becton Dickinson, cat. no. 309628) DNA origami preparation Thermocycler, MiniAmpTM Thermal Cycler REX (Thermo Fisher Scientific, cat No. A38080) PCR tubes (USA Scientific, cat. no. 1402-8120) 100 kD amicon filter (Milipore, cat. no. UFC510024) Benchmark MC-12 High Speed Microcentrifuge (Marshall Scientific, item no. C1612) Rate-zonal centrifugation Beckman TLS 55 swinging bucket rotor (Beckman Coulter, part no. 346936) Polycarbonate centrifuge tube (Beckman Coulter, part no. 343778) Optima TLX ultracentrifuge (Beckman Coulter, part no. 361545) Longneck gel-loading tips (Fisher Scientific, cat. no. 02-707-81) Safe Imager 2.0 Blue Light transilluminators (Invitrogen, cat. no. G6600) Gel electrophoresis 250 mL Erlenmeyer flask (Karter Scientific, part no. 214U2) External gel cast (Thermo Fisher Scientific, cat. no. B2-CST) OwlTM easyCastTM B2 Mini Gel Electrophoresis Systems (Thermo Fisher Scientific, cat. no. B2-BP) Bio-Rad Molecular Imager Gel Doc XR+ (Bio-Rad, part. no. 1708195EDU) Atomic force microscopy MultiMode 8-HR AFM (Bruker) ScanAsyst-Fluid+probes cantilever (Bruker) Glass probe holder MTFML-V2 (Bruker) V1 AFM mica discs, 10mm (Ted Pella Inc., product no. 50-10) AFM/STM Metal Specimen Discs, 12mm (Ted Pella Inc., product no. 16208) ..
Microscopy:Article Title: Gradient-mixing LEGO robots for purifying DNA origami nanostructures of multiple components by rate-zonal centrifugation
Article Snippet: Concentration gradient 50 × TAE buffer, pH 8.3 (VWR Life Science, cat. no. 75800-940) Magnesium chloride hexahydrate (Sigma-Aldrich, cat. no. M9272) Glycerol (VWR Life Science, cat. no. BDH1172) Sucrose (Sigma-Aldrich, product no. S 0389) DNA origami folding 50× TAE buffer, pH 8.3 (VWR Life Science, cat. no. 75800-940) Magnesium chloride hexahydrate (Sigma-Aldrich, cat. no. M9272) Staple strands (Integrated DNA Technique Inc.) ssDNA scaffold p8064 (Tilibit Nanosystem, product no. M1-51) Agarose Gel Electrophoresis SYBR gold DNA gel stain (Thermo Fisher Scientific, cat no. S11494) DNA gel loading dye (Thermo Fisher Scientific, cat no. R0631) 1 kb DNA ladder (Gold Biotechnology Inc., cat. no. D010-500) Agarose tablets (EURx, cat. no. E0305-01) .. General P2L Eppendrof pipette (Gilson, SKU FA10001M) P10L Eppendrof pipette (Gilson, SKU FA10002M) P200L Eppendrof pipette (Gilson, SKU FA10005M) P1000L Eppendrof pipette (Gilson, SKU FA10006M) EXPERT tips E200 Tipack (Gilson, SKU F1733002) EXPERT Tips E1000 XL Tipack (Gilson, SKU F1735002) Milli-Q® Direct Water Purification System (Milipore Sigma, cat. no. ZR0Q016WW) Density gradient preparation EV3 LEGO Mindstorm (LEGO, item no. 31313) Ultimaker 3 3D printer (part no. 9671) PLA 3D printing materials 1 mL syringe (Becton Dickinson, cat. no. 309628) DNA origami preparation Thermocycler, MiniAmpTM Thermal Cycler REX (Thermo Fisher Scientific, cat No. A38080) PCR tubes (USA Scientific, cat. no. 1402-8120) 100 kD amicon filter (Milipore, cat. no. UFC510024) Benchmark MC-12 High Speed Microcentrifuge (Marshall Scientific, item no. C1612) Rate-zonal centrifugation Beckman TLS 55 swinging bucket rotor (Beckman Coulter, part no. 346936) Polycarbonate centrifuge tube (Beckman Coulter, part no. 343778) Optima TLX ultracentrifuge (Beckman Coulter, part no. 361545) Longneck gel-loading tips (Fisher Scientific, cat. no. 02-707-81) Safe Imager 2.0 Blue Light transilluminators (Invitrogen, cat. no. G6600) Gel electrophoresis 250 mL Erlenmeyer flask (Karter Scientific, part no. 214U2) External gel cast (Thermo Fisher Scientific, cat. no. B2-CST) OwlTM easyCastTM B2 Mini Gel Electrophoresis Systems (Thermo Fisher Scientific, cat. no. B2-BP) Bio-Rad Molecular Imager Gel Doc XR+ (Bio-Rad, part. no. 1708195EDU) Atomic force microscopy MultiMode 8-HR AFM (Bruker) ScanAsyst-Fluid+probes cantilever (Bruker) Glass probe holder MTFML-V2 (Bruker) V1 AFM mica discs, 10mm (Ted Pella Inc., product no. 50-10) AFM/STM Metal Specimen Discs, 12mm (Ted Pella Inc., product no. 16208) ..
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